Journal: Nature Communications

Article Title: Brown adipose tissue secretes OLFM4 to coordinate sensory and sympathetic innervation via Schwann cells

doi: 10.1038/s41467-025-60474-1

Figure Lengend Snippet: Olfm4 flox/flox mice with Ucp1 -Cre mice were crossed to construct brown adipocyte-specific knockout mice ( Ucp1 -Cre + ; Olfm4 flox/flox , Olfm4CKO) for Olfm4 , and littermate Cre-negative mice ( Ucp1 -Cre - ; Olfm4 flox/flox , Ctrl) served as controls. Ctrl and Olfm4CKO mice (8 weeks old) were treated with normal diet (ND), Body weight was recorded ( n = 6 mice per group) ( A ). General representative image and weight of Ctrl and Olfm4CKO iBAT ( B ). Hematoxylin-eosin (HE) staining of Ctrl and Olfm4CKO iBAT ( n = 6 mice per group) ( C ) (scale bars, 50 μm and 20 μm). Triglyceride quantification of Ctrl and Olfm4CKO mice ( n = 6 mice per group) ( D ). Indirect calorimetry analysis of Ctrl and Olfm4CKO mice, monitored over a 24 h period ( n = 3 mice per group) ( E ). Daily food intake of Ctrl and Olfm4CKO mice ( n = 6 mice per group) ( F ). Ctrl and Olfm4CKO mice were cold challenged at 4 °C. Rectal temperatures are shown ( n = 6 mice per group) ( G ). Ctrl and Olfm4CKO mice (8 weeks old) were treated with an high-fat diet (HFD) for 10 weeks. Body weight was recorded each week ( n = 6 mice per group) ( H ). Tissues were collected and weighed. Fat mass of Ctrl and Olfm4CKO mice ( n = 6 mice per group) ( I ). Lean mass of Ctrl and Olfm4CKO mice ( n = 6 mice per group) (J). Intraperitoneal glucose tolerance test (GTT) ( n = 6 mice per group) ( K ). Histology of iBAT ( L ) and liver ( M ) of Ctrl and Olfm4CKO mice on an HFD (scale bars, 50 μm and 20 μm). All Data are mean ± s.e.m. of biologically independent samples. Statistical analysis was performed using two-tailed unpaired Student’s t -test ( A − J ) and two-tailed unpaired Welch’s t -test ( G − K ). All experiments were independently repeated at least three times with similar results. Source data are provided as a Source Data file.

Article Snippet: The concentration of triglycerides in the serum was quantified with a serum triglyceride determination kit (#98-11086-01, IDEXX, Westbrook, USA) and a seralyzer (Catalyst Dx TM , IDEXX, Westbrook, USA).

Techniques: Construct, Knock-Out, Staining, Two Tailed Test

Journal: Advanced healthcare materials

Article Title: Effect of Donor Age and Liver Steatosis on Potential of Decellularized Liver Matrices to be Used as a Platform for iPSC-Hepatocyte Culture

doi: 10.1002/adhm.202302943

Figure Lengend Snippet: A. The pictures and H&E staining of livers with no steatosis, low steatosis, and high steatosis before decellularization. B. The pictures, H&E staining, and oil red o staining of livers with no steatosis, low steatosis, and high steatosis after decellularization. C. The triglyceride content (mg triglyceride per mg tissue) of livers before (T=0) and after (decellularized) decellularization as well as after pepsin digestion (digested) and filtration (filtered). D. The collagen content, and E. the GAG content of no steatosis, low steatosis, and high (T=0) and after (decellularized) decellularization. (Scale bars = 200 μm) (Statistical significance is shown by * p<0.05, ** p<0.01, ***p<0.001) ($ indicates statistical significance when compared to no steatosis hDLM, and # indicates statistical significance when compared to low steatosis hDLM) (Student’s t-test, n = 3).

Article Snippet: The triglyceride (TGA) content of no steatosis, low steatosis, and high steatosis livers before and after decellularization, as well as that of iPSCs differentiated to IHC and HM stages on the corresponding hDLMs was determined using Serum Triglyceride Quantification Kit (Cell Biolabs, Inc., STA-396) following manufacturer’s instructions.

Techniques: Staining, Filtration

Journal: Advanced healthcare materials

Article Title: Effect of Donor Age and Liver Steatosis on Potential of Decellularized Liver Matrices to be Used as a Platform for iPSC-Hepatocyte Culture

doi: 10.1002/adhm.202302943

Figure Lengend Snippet: Quantification of A. albumin secretion, B. urea secretion, C. cellular triglyceride content of iPSC-hepatocytes at IHC and HM stages when differentiated on geltrex, no steatosis, low steatosis, and high steatosis hDLM. The qRT-PCR analysis showing the mRNA expression of fat metabolism markers ACACA, ACSL4, FASN, and FABP5 in D. iPSC-hepatocytes at HM stage, and E. in primary human hepatocytes when cultured on no steatosis, low steatosis, and high steatosis hDLM relative to geltrex and collagen, respectively. (Statistical significance is shown by * p<0.05, ** p<0.01, ***p<0.001) (# represents significant difference (p<0.05) compared to high fat hDLM at IHC stage) (Student’s t-test, n = 3).

Article Snippet: The triglyceride (TGA) content of no steatosis, low steatosis, and high steatosis livers before and after decellularization, as well as that of iPSCs differentiated to IHC and HM stages on the corresponding hDLMs was determined using Serum Triglyceride Quantification Kit (Cell Biolabs, Inc., STA-396) following manufacturer’s instructions.

Techniques: Expressing, Quantitative RT-PCR, Cell Culture